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Instructions 71-5000-15 AD Pre-activated media
GE Healthcare
CNBr-activated
Sepharose 4 Fast Flow
Introduction
The preparation and use of affinity chromatography media by coupling biospecific
ligands to CNBr-activated matrices is a widely used, successful and well-documented
technique.
CNBr-activated Sepharose
TM
4 Fast Flow is a pre-activated affinity matrix that
combines the advantages of CNBr coupling with the high flow and stability
characteristics of Sepharose 4 Fast Flow. In our experience, the CNBr coupling
technique has a well-proven track record for the purification of therapeutic
proteins. This, plus the performance of the matrix at large scale, makes the use of
CNBr-activated Sepharose 4 Fast Flow particularly attractive for manufacturing
applications in the biopharmaceutical industry. Furthermore, the medium is a
member of the BioProcess
TM
media. BioProcess media are developed and supported
for production scale chromatography. All BioProcess media are produced with
validated methods and are tested to meet manufacturing requirements. Secure
ordering and delivery routines give a reliable supply of media for production
scale. Regulatory Support Files (RSF) are available to assist process validation and
submissions to regulatory authorities.
To ensure best performance and trouble-free operation, please read these
instructions before using CNBr-activated Sepharose 4 Fast Flow.