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p. 11
or
Wash the column with 3– column volumes of 70%
ethanol. Wash immediately with at least 5 column
volumes of sterile filtered binding buffer.
Sanitization
Sanitization inactivates microbial contaminants in the packed column and
related equipment. A specific sanitization protocol should be designed for
each process according to the type of contaminants present and stability of
coupled ligand. Following are generally recommended procedures.
Equilibrate with a buffer consisting of 2% hibitane digluconate and 20%
ethanol. Allow to stand for 6 hours, then wash with at least 5 column
volumes of sterile binding buffer.
or
Equilibrate with 70% ethanol. Allow to stand for 12 hours, then wash with at
least 5 column volumes of sterile binding buffer.
Note: Specific regulations may apply when using 70% ethanol since it can
require the use of explosion-proof areas and equipment.
Storage
CNBr-activated Sepharose 4 Fast Flow is supplied freeze dried. Additives are
included to preserve the bead form of the medium. When stored below 8 °C,
the shelf life is at least 18 months. Packed columns should preferebly be
equilibrated in binding buffer containing 20% ethanol (ligand dependent) to
prevent microbial growth.
6. Ordering information
Product Pack size Code No
CNBr-activated Sepharose 4 Fast Flow 10 g 17-0981-01
250 g 17-0981-03
2 kg 17-0981-05
All products are supplied freese dried in the precence of additives.