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7. Scaling up
For quick scale-up of purifications (back pressure will increase), two or three
HiTrap ion exchange columns of the same type can be connected in series.
For further scale-up SP Sepharose High Performance and Q Sepharose High
Performance are available in prepacked HiLoad
™
columns or bulk media
packs. See Ordering Information.
8. Storage
HiTrap SP HP: Rinse with water and then wash with 5 column volumes of
20% ethanol, 0.2 M sodium acetate.
HiTrap Q HP: Rinse with water and then with 5 column volumes of 20%
ethanol.
Seal the column with the supplied stoppers. The recommended storage
temperature is +4 to +30 °C.
9. Ordering Information
Product No. Supplied Code No.
HiTrap SP HP 5 x 1 ml 17-1151-01
HiTrap SP HP 5 x 5 ml 17-1152-01
HiTrap Q HP 5 x 1 ml 17-1153-01
HiTrap Q HP 5 x 5 ml 17-1154-01