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F-801 Internal RO System by GE Osmonics
12552e – 27 Feb 04 4- 30 Routine Maintenance
4.5.1 Product Water Culture Procedure
This procedure assumes the RO is connected to power and water, and is in operating
condition.
1. Close the product valve and connect the extension hose to the product line. .
Place the other end in a drain and open the product valve.
2. Turn on the RO unit. Allow the RO unit to run for 10-15 minutes.
3. Close the product valve on the product line and remove the extension hose.
4. Aseptically clean inside and outside of the product line quick disconnect fitting
with three Betadyne swabs (optional).
5. Using aseptic technique, hold the product line over a container, and allow
approximately 100 cc of product (RO) water to flow to the drain (or allow
product water to flow for 30 seconds).
6. Maintaining aseptic technique, hold the product line over the sterile container
with the product water flowing.
7. Fill the container with RO product water. (If using commercial samplers, follow
the manufacturer's instructions.)
8. Aseptically cap the specimen container immediately.
9. Label the specimen appropriately with:
• Test to be performed - "culture / colony count"
• Sample source - product water/RO, machine serial number and room
location
• Time and date sample obtained
• Person who obtained specimen
• Any other pertinent information or procedures your facility or lab requires
10. The samples should be assayed within 30 minutes of obtaining or refrigerated at
5° C immediately and assayed within a 24-hour period.