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RPN2510EUM Pagefinder Rev C 06/2007 2
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1. Legal 3
2. Introduction 4
3. Safety warnings and precautions 5
3.1. Electrical Installation 5
4. Specification 6
5. Setting up the Hybridization Oven/Shaker 7
5.1. Setting the oven temperature 7
5.2. Setting up the rotisserie 7
5.3. Setting up the platform shaker 8
6. Hybridization using the platform shaker 9
7. Hybridization using the rotisserie 10
7.1. Assembly of membranes into bottles 10
7.2. Hybridization 10
7.3. Membrane washing procedures 11
7.3.1. Radioactive Hybridizations 11
7.3.2. AlkPhos Direct Hybridizations 11
8. Maintenance/care/cleaning of the oven/shaker 12
8.1. Error codes/faults 12
9. Troubleshooting guide 13
Appendix 1. Products for electrophoresis 14
Appendix 2. Hybond membranes for nucleic acid applications 15
Appendix 3. Radioactive labeling systems 16
Appendix 4. Non-radioactive labeling and detection systems 17
Appendix 5. Products for autoradiography and chemiluminscent
detection 18
Appendix 6. Hybridization buffer 19
Appendix 7. Radiation safety products 20