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5. Setting up the Hybridization
Oven/Shaker
RPN2510EUM Chapter 5 Rev C 06/2007 7
Fig 1. Diagram of instrument control
panel
Rotisserie/shaker controls
1. Rotisserie/shaker ON/OFF button
2. Rotisserie/shaker speed selector dial
3. Motor on indicator
Temperature controls
4. Temperature on/off button
5. Press to set button
6. LED temperature display
7. Temperature selector dial
Note: the Oven is fitted with automatic
digital over-temperature protection (see
Section 8.1. for more details).
NOTE: Always ensure that the weight
is evenly distributed on both sides
of the rotisserie. Place an empty
hybridization bottle into the other side
of the rotisserie as a counterbalance if
necessary.
Hybridisation Oven/Shaker
press
to set
Rotisserie
rotisserie
(rev/minute)
5
10
70
15
shake r
(strokes/minute)
8
60
2
30
4
45
6
0
Te mperatur e (
°C )
on
off
on
off
1 2 3 4 5 6 7
1. Remove all packaging and place the Hybridization Oven/Shaker on a level
working surface, ensuring that there is sufficient room above the instrument to
allow the door to be opened fully.
2. Leave the unit to stand for a minimum of 3 hours. This will allow acclimation to
the new ambient temperature.
3. Plug the female end of the power cable into the Hybridization Oven/Shaker.
4. Connect the power cable to a suitably grounded electrical outlet. The correct
operating voltage of the Hybridization Oven/Shaker is found on the product
information label on the rear of the instrument.
5. Turn the Mains ON/OFF switch (1 on Fig 1) to the ON position.
6. The GE Healthcare Hybridization Oven/Shaker is now ready for use.
5.1. Setting the oven temperature
1. The Oven temperature controls are located on the right hand side of the control
panel (numbers 4 to 7 in figure 1).
2. To turn the temperature control ON press the on / off button (4). The LED display
(6) will show the current Oven temperature.
3. To set the temperature, press and hold the press to set button (5) and at
the same time rotate the temperature selector dial (7) until the required
temperature is shown on the digital display (6).
4. Release the press to set button (5) and the temperature display (6) will revert
back to the actual Oven temperature.
5. The set temperature can be viewed at any time simply by pressing and holding
the press to set button (6)
6. The oven will now automatically heat up to the set temperature.
5.2. Setting up the rotisserie
The rotisserie is installed in the Hybridization Oven/Shaker during transit. To use
the rotisserie for bottle hybridization, the following procedure should be adopted:
1. Lift up the oven door to its fullest extent to allow complete access to the oven
interior.
2. Lift the rotisserie vertically out of the oven. Unpack and place on the bench.
3. Place the membranes to be hybridized into the required number of hybridization
bottles. Using the rotisserie as a stand for the bottles, place the bottles into the
rotisserie, pushing them down as far as they will go.