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Table 4. Prepacked columns for desalting and buffer exchange.
Code No Column Loading volume Elution volume Comments Application
17-1408-01 HiTrap 0.1–1.5 ml 1.3–4.0 ml Prepacked with For desalting and buffer
Desalting Sephadex™ G-25 exchange of protein
Superfine. Requires extracts (M
r
>5000).
a syringe or pump
to run.
17-5087-01 HiPrep Up to 15–20 ml Prepacked with For desalting and
26/10 15 ml Sephadex G-25 buffer exchange of
Desalting Fine. Requires a protein extracts
pump to run. (M
r
>5000).
17-0851-01 PD-10 2.5 ml 3.5 ml Prepacked with For desalting and
Desalting Sephadex G-25. buffer exchange
Requires only of protein extracts
gravity to run. (M
r
>5000).
17-0855-01 NICK™ 0.1 ml 0.4 ml Prepacked with For separation of
Sephadex G-25. proteins (M
r
>5000)
and nicktranslated
DNA from radiolabelled
nucleotides not shorter
than 120 mers, and
similar separations.
17-0853-01 NAP™-5 0.5 ml 1.0 ml Prepacked with For purification of
17-0854-01 NAP-10 1.0 ml 1.5 ml Sephadex G-25 proteins (M
r
>5000),
17-0852-01 NAP-25 2.5 ml 3.5 ml DNA grade. DNA and oligo-
Requires only nucleotides greater
gravity to run. than 10 bases in length.