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Fig 3. Recommended buffer substances for anion exchange chromatography.
The columns can be operated with a syringe, peristaltic pump or a
chromatography system.
3. Operation
Buff er preparation
Water and chemicals used for buffer preparation should be of high purity. It
is recommended to filter the buffers by passing them through a
0.22 μm filter immediately before use. See Tables 2 and 3, Figs 2 and 3 for
recommended buffers.
Sample preparation
The sample should be adjusted to the composition of the start buffer.
This can be done by either diluting the sample with start buffer or by
buffer exchange using HiTrap Desalting, HiPrep
™
26/10 Desalting or PD-10
column. The sample should be filtered through a 0.22 μm or 0.45 μm filter or
centrifuged immediately before it is applied to the column. See Table 4.