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Table 3. Buffers for anion exchange chromatography.
pH interval Substance Conc. (mM) Counter-ion pKa (25 °C)
1
4.3–5.3 N-Methylpiperazine 20 Cl
-
4.75
4.8–5.8 Piperazine 20 Cl
-
or HCOO
-
5.33
5.5–6.5 L-Histidine 20 Cl
-
6.04
6.0–7.0 bis-Tris 20 Cl
-
6.48
6.2–7.2; 8.6–9.6 bis-Tris propane 20 Cl
-
6.65; 9.10
7.3–8.3 Triethanolamine 20 Cl
-
or CH
3
COO
-
7.76
7.6–8.6 Tris 20 Cl
-
8.07
8.0–9.0 N-Methyldiethanolamine 20 SO
4
2-
8.52
8.0–9.0 N-Methyldiethanolamine 50 Cl
-
or CH
3
COO
-
8.52
8.4–9.4 Diethanolamine 20 at pH 8.4 Cl
-
8.88
50 at pH 8.8
8.4–9.4 Propane 1,3-Diamino 20 Cl
-
8.88
9.0–10.0 Ethanolamine 20 Cl
-
9.50
9.2–10.2 Piperazine 20 Cl
-
9.73
10.0–11.0 Propane 1,3-Diamino 20 Cl
-
10.55
10.6–11.6 Piperidine 20 Cl
-
11.12
1
Ref: Handbook of chemistry and physics, 83
rd
edition, CRC, 2002–2003.
pH
Fig 2. Recommended buffer substances for cation exchange chromatography.