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Table 1. HiTrap SP HP and HiTrap Q HP columns characteristics
Column volumes 1 ml or 5 ml
Column dimensions 0.7 × 2.5 cm (1 ml) and 1.6 × 2.5 cm (5 ml)
Total ionic capacity 0.14–0.20 mmol (Cl
–
)/ml medium (Q)
0.15–0.20 mmol (H
+
)/ml medium (SP)
Dynamic binding capacity SP: approx. 55 mg ribonuclease/ml medium
(0.1 M sodium acetate, pH 6.0 at 1 ml/min)
Q: approx. 50 mg HSA/ml medium (20 mM Tris-HCl,
pH 8.2 at 1 ml/min)
Mean particle size 34 μm
Bead structure 6% highly cross-linked spherical agarose
Maximum flow rates HiTrap 1 ml: 4 ml/min, HiTrap 5 ml: 20 ml/min
Recommended flow rates HiTrap 1 ml: 1 ml/min, HiTrap 5 ml: 5 ml/min
Maximum backpressure 0.3 MPa, 3 bar, 42 psi
Chemical stability All commonly used buffers
Charged group SP: – CH
2
CH
2
CH
2
SO
3
Q: – CH
2
N
+
(CH
3
)
3
pH stability*
Short term SP: 3–14, Q: 1–14
Working SP: 4–13, Q: 2–12
Long term SP: 4–13, Q: 2–12
Storage temperature +4° to +30 °C
Storage buffer SP: 20% ethanol, 0.2 M sodium acetate
Q: 20% ethanol
Avoid SP: Oxidizing agents, cationic detergents and
buffers
Q: Oxidizing agents, anionic detergents and
buffers
* The ranges given are estimates based on our knowledge and experience.
Please note the following:
pH stability, long term refers to the pH interval where the medium is stable over
a long period of time without adverse eff ects on its subsequent chromatographic
performance.
pH stability, short term refers to the pH interval for regeneration, cleaning-in-place and
sanitization procedures.