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Appendix 1. Products for
electrophoresis
DNA Markers
Precise Sizing Digest of Natural DNAs
50 Base-pair 100 Base-pair KiloBase DRigest III
ladder ladder DNA marker 27-4060-01
27-4005-01 27-4007-01 27-4004-01
500
a
2000
a
10000 23130
b
450 1900 8000 9416
400 1800 6000 6557
350 1700 5000 4361
b
300 1600 4000 2322
250 1500 3000 2027
200 1400 2500 1353
150 1300 2000 1078
100 1200 1500 872
50 1100 1000 603
1000 500 564
d
900 310
800 281
700 271
600 234
500 194
400 125
c
300 118
200 72
100
a not necessarily the largest size fragment possible, only the largest that is
readily distinguishable
b cos ends are located on these bands
c may not always be visible
Marker code number Recommended gel Loading amount (µg/lane) Heat before loading
27-4005-01 2% agarose/6% polyacrylamide 2.0 No
27-4007-01 1.5% agarose 2.0 No
27-4004-01 0.8% agarose 0.5 No
27-4060-01 1% agarose 0.5–1.0 60–65°C for 2 minutes
RPN2510EUM Appendix 1 Rev C 06/2007 14