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Appendix 3. Radioactive labeling
systems
Labelling Technology Nucleotide Amount of Labelling Probe specific Recommended Product
system template time activity application code
(dpm/µg)
Rediprime
™
II Random-prime dCTP only 25 ng 10 minutes 2
×
10
9
membrane RPN1633
hybridization RPN1634
Ready-To-Go
™
Random-prime dCTP only 10 ng–1 µg 5 minutes 2
×
10
9
membrane 27-9240-01
DNA labelling hybridization
beads DNA labelling
Megaprime
™
Random-prime any dNTP 25 ng 10 minutes 2
×
10
9
membrane RPN1604
hybridization RPN1605
RPN1606
RPN1607
Nick translation Nick translation any dNTP 1 µg 2–3 hours 2
×
10
9
production of N5000
large amounts N5500
of probe
RPN2510EUM Appendix 3 Rev C 06/2007 16