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Appendix 4. Non-radioactive
labeling and detection systems
Labelling and detection Sensitivity Time from Duration of Strip before Quantification Recommended application
system hybridization light output re-probing
to detection
AlkPhos Direct (RPN3690) 0.06 pg 1 hour 5 days yes no Single copy Southern and
Northerns
ECL Direct (RPN3000) 0.5 pg 1 hour 1–2 hours no no High target applications
eg. colony/plaques
RPN2510EUM Appendix 4 Rev C 06/2007 17