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Creating a New Wash Protocol
Specifying Wash Cycle Operations
The final step in creating a wash protocol is to specify individual operations
within the wash protocol.
An example of a wash protocol is shown in 45. In this example, the
dispense
wash
pin
s are purged, and then a Dispense and Aspirate are carried out
twice on each of the 12 strips (columns) of a 96-well Microtiter
®
plate.
Finally, after all 12 strips have been washed twice, the contents of the
Microtiter plate are allowed to soak (equilibrate) for another 600 seconds.
PURGE Purge the dispense pins.
↓
MOVE
(2 Cycles)
Specify the starting strip and ending strip for each cycle
and the number of cycles to be performed.
↓
DISPENSE
(3 Times)
For each strip, perform dispense three times.
↓
ASPIRATE For each strip, perform one aspiration cycle.
↓
END LOOP End the cycle.
↓
SOAK
After the two Move cycles have been completed, allow
the contents to equilibrate for 600 seconds.
↓
END End the wash protocol.
Figure 9. Example of a Wash Protocol
The procedures for specifying the steps in this and other wash protocols are
described on the following pages.
Opsys MW™ User’s Guide 45