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Operational Problems
Symptom Probable Cause Resolution
Dispense volumes
are incorrect.
The dispense
pins are leaking.
Check the pins, seals and
tubing for leaks.
The dispense
pressure is low.
Check the seals and
tubing for leaks.
Check for air leaks.
Reseat the tubing in the
dispense valve.
The dispense
pins are clogged.
Remove the wash head
assembly and clean it
using the manifold head
cleaning wire. Then run a
purge. Service is required
if problem persists.
Incorrect
Calibration
Recalibrate Pump
Aspiration is not
complete.
The aspiration
pins are clogged.
Remove the wash head
assembly and clean it
using the manifold head
cleaning wire. Service is
required if problem
persists.
The aspiration pin
height is set too
high.
Repeat the plate setup
procedure (page 30).
The seal on the
waste container is
leaking.
Check the seal and waste
tubing for leaks.
The wash
protocol is not
optimized.
If the bottoms of the plate
wells are flat, use a
sweep.
Add additional aspiration
cycles to the wash
protocol.
Aspiration tubing
may be blocked
or kinked.
Check the aspiration
tubing and remove any
blockages or kinks.
The vacuum
exhaust may be
blocked.
Remove any obstructions
at the vacuum exhaust
(see page 17).
The vacuum
pump may be
contaminated by
overflow.
Service is required.
Opsys MW™ User’s Guide 79