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15
Programming Options
There are two options available for creating programs on the CYCLER:
1. Copy and edit one of the pre-written templates.
2. Create a new program from the defaults.
Option 1: Creating programs using templates
There are two pre-written basic PCR templates: a two-step and a three-step template. These can
be copied and modified as required. "In denat" stands for Initial Denaturing.
Other templates available include Ice bucket (incubation at 4ºC), Ligation (incubation at 15ºC) and
an RT-PCR template.
Use the “Down Arrow” key to select an
existing ‘Template’ program from the
menu, e.g. ‘2 Step Template’ or ‘3 Step
Template’.
Press the “Enter” key to accept it.
The Template is already defined and can
be run as it is or easily copied, modified
and saved under a new name.
* * New progr a
2 STEP TEMPLATE 501
3 STE
[ , to select ]
-*New*-
2 STEP
3 STEP
[ , to select ]
2 STEP [2]
3 STEP 3
[ , to select ]
[ , to select ]
2 STEP TEMPLATE [501]
Run Program
View Program
[ , to select ]
2 STEP
[
2
]
Run Method
View Method
[ , to select ]
[ , to select ]
2 STEP TEMPLATE
Heated Lid 105 º
C
Preheat Lid on
Pause off
- - - - - - - - - - -
In denat
9
4.0
º
C 5m00
Hot Start off
- - -
Stage 1
- - - -
Number cycles 30
Step 94.0 º
C
0m30 max
Step 68.0 º
C
1m00 max
- - - - - - - - - - -
Fina Extn
7
2.0 º C 5m00
Final hold 10.0
ºC
2 STEP
Heated Lid 105 º
C
Preheat Lid on
Pause off
- - - - - - - - - - -
In denat
9
4.0
º
C 5m00
Hot Start off
- - -
Stage 1
- - - -
Number cycles 30
Step 94.0 º
C
0m30 max
Step 68.0 º
C
1m00 max
- - - - - - - - - - -
Post run
7
2.0 º C 5m00
Post hold 10.0
ºC
3
STEP TEMPLATE
H
eat
e
d Lid 105 º C
P
reheat Lid on
P
ause off
-
-
-
-
-
-
-
-
- - -
I
n
d
enat
9
4.0
º
C 5m00
H
ot Start off
- - -
S
tage 1
- - - -
N
umber cycles 30
S
tep 94.0
º
C
0
m30 max
S
tep 55.0
º
C
0
m30 max
S
tep 72.0
º
C
0
m30 max
-
-
-
-
-
-
-
-
- - -
F
inal
E
xtn
7
2.0
º
C 5m00
F
inal hold 10.0 º C
3
STEP
H
eated Lid 105 º C
P
reheat Lid on
P
ause off
-
-
-
-
-
-
-
-
- - -
I
n
d
enat
9
4.0
º
C 5m00
H
ot Start off
- - -
S
tage 1
- - - -
N
umber cycles 30
S
tep 94.0
º
C
0
m30 max
S
tep 55.0
º
C
0
m30 max
S
tep 72.0
º
C
0
m30 max
-
-
-
-
-
-
-
-
- - -
P
ost
r
un
7
2.0
º
C 5m00
P
ost hold 10.0 º C