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Example programs
A couple of example thermal cycling programs are shown below.
Typical 3-Step PCR
Heated Lid 105
Preheat Lid on
Pause off
- - - - - - - - - - - - - - - -
In denat 94°c 5min
Hot Start off
- - - - - - Stage 1 - - - - - -
Number Cycles 30
Step 94°c 0m30 max
Step 55°c 0m30 max
step 72°c 0m30 max
- - - - - - - - - - - - - - - -
Post run 72°c 5m
Post hold 4ºC 10m
3-Step PCR with multiple ‘Stages’
Heated Lid 105
Preheat Lid on
Pause off
- - - - - - - - - - - - - - - -
In denat 94
°c 5min
Hot Start off
- - - - - - Stage 1 - - - - - -
Number Cycles 10
step 94
°c 0m30 max
step 60
°c 0m30 max
step 72
°c 0m30 max
- - - - - - Stage 2 - - - - - -
Number Cycles 20
step 94
°c 0m30 max
step 55
°c 0m30 max
step 72
°c 0m30 max
- - - - - - - - - - - - - - - -
Post run 72
°c 5m
Post hold 4ºC 10m
Programming
Create a new program by selecting
-* New *- from the menu and press “Enter”
Give the program a name and edit any of
the default fields as described above.
To add a stage and then edit it
Use the “Down Arrow” key to scroll to
the dotted line below ‘Hot Start’; it will
start to flash.
Press "Enter" to insert a stage
* * New program ** []
2 STEP TEMPLATE 501
3 STEP TEMPLATE 502
[ , to select ]
-*New*-
[
]
2 STEP 2
3 STEP 3
[ , to select ]