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The amount of volume loss you observe and the change in reactant concentrations you can
tolerate determine the minimum volume that can be used. Typical volume losses of 1µl in 30
cycles allow the use of samples of 20µl or less.
During the final cool-down, a ring of condensation may form above the liquid level but below the
top of the sample block. This is normal and does not effect the reaction.
0.5ml Tubes
The heated lid mechanism of the Cycler TCY with the 0.5ml block is optimised for flat top tubes. In
order to fit the maximum number of tubes in the block, they need to be placed so that all the
hinges are in the same relative orientation. An example is illustrated below.
Place the tubes in the block in the same
orientation in order to achieve the maximum
capacity.
Always make sure the pressure on the
heated lid is balanced by spreading the tubes
across the block or inserting some dummy
tubes if necessary.