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4-12
Operation - Standard Use 4
Essential Steps
In Run Screen (1)
1
air gap
2
liquid
3
fraction1
4
fraction 2
5
fraction 3
6
fraction 4
7
to waste
Extra Volume
In Run Screen (2a)
In Run Screen (2b)
Ensure that the probe-end of the transfer-tubing is
in a suitable waste container, then press OK.
‘Homing ...’ appears on the screen until the
syringe(s) have returned to their home positions,
during which time any liquid contained in the
transfer-tubing is expelled from the probe-end.
After this the Action Screen reappears.
Press ESC to return to the start-up screen for the
current file.
Whenever you see ‘Homing’ on the screen, the
syringe piston(s) are returned to their uppermost
position(s). As a result, some (or all) of the liquid
contained in the transfer-tubing will be expelled
from the probe-end. You must ensure that the
probe end of transfer-tubing is already in a
suitable waste container.
Method Example
This section describes how to run Method 33
(created as above).
(i) Press Run, you see In Run Screen 1.
M33/T 1: DISPENS/1 means Method 33 Task 1,
Dispense Task, first cycle.
The cursor moves from side-to-side below the
parameter value field, indicating that you must
enter a value (or validate the current value, in
this example 6 mL/min.). Key in the required
flow rate (for example, 12 mL/min.) and press
ENTER. [Similar screens would appear for any
other parameters that you had entered as a
question mark (?).] The command light flashes
and you see alternately In Run Screens 2a and 2b
(about every half a second).
In the Dispense task, you aspirate an air gap, then
liquid. After aspirating, you dispense the liquid
in several fractions, finally the ‘Extra volume’ is
sent to waste.